Micro immuno supported liquid membrane (μ-ISLM) extraction

A mu-ISLM (micro Immuno Supported Liquid Membrane) extraction system coupled in a sequential injection analysis (SIA) configuration, capable of simultaneous sample cleanup and enrichment was developed. The capacity of the extraction channels - donor and acceptor, respectively - were reduced from originally 10 to 1.65 mu L. The acceptor channel surface was covered with a gold layer enabling immobilisation of antibodies via an alkanthiol self-assembled monolayer. The mu-ISLM-SIA system resulted in very high extraction efficiency and enrichment factor (Extraction efficiency (E): 136%; Enrichment factor (E-e): 544) and a subsequent low detection limit for the model analyte simazine of 0.1 ng/L.