Identification of Aeromonas isolates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry

被引：29
|
作者：
Lamy, Brigitte [1 ,2 ,3 ]
Kodjo, Angeli [4 ]
Laurent, Frederic [2 ,5 ,6 ]
机构：
[1] Ctr Hosp Univ Montpellier, Bacteriol Lab, Hop Arnaud Villeneuve, F-34059 Montpellier, France
[2] Virol & Hyg Hopitaux Generaux, ColBVH, Coll Bacteriol, Le Chesnay, France
[3] Univ Montpellier 1, UMR 5119, Montpellier, France
[4] Univ Lyon 1, CNRS, Univ Lyon, Ecol Microbienne,UMR 5557, F-69622 Villeurbanne, France
[5] Hop Croix Rousse, Hospices Civils Lyon, Bacteriol Lab, F-69317 Lyon, France
[6] Univ Lyon 1, INSERM, U851, F-69365 Lyon, France
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2011年 / 71卷 / 01期
关键词：
Aeromonas; Bacterial identification; MALDI-TOF MS; Evaluation; Accuracy; GENUS AEROMONAS; PHENOTYPIC IDENTIFICATION; ROUTINE IDENTIFICATION; BACTERIA; MICROORGANISMS; STRAINS;
D O I：
10.1016/j.diagmicrobio.2011.04.014
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We evaluated the accuracy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying aeromonads with an extraction procedure. Genus-level accuracy was 100%. Compared to rpoB gene sequencing, species-level accuracy was 90.6% (29/32) for type and reference strains and 91.4% for a collection of 139 clinical and environmental isolates, making this system one of the most accurate and rapid methods for phenotypic identification. The reliability of this technique was very promising, although some improvements in database composition, taxonomy, and discriminatory power are needed. (C) 2011 Elsevier Inc. All rights reserved.
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页码：1 / 5
页数：5
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