Identification and growth-promoting effect of endophytic bacteria in potato

In agriculture, Bacillus species are efficient and ecologically tool for promote the growth of the plant. Purpose: This study obtains the plant growth-promoting (PGP) ability of endophytic bacteria isolated from the potato tubers. Methods: Using endophytic bacteria to promote potato growth, achieve the purpose of increasing production. In this experiment, the growth- promoting ability of the strain was verified by laboratory identification and field test validation. Result: The isolates were identified as Bacillus species based on a 16S rRNA gene sequence and gyrB gene sequence analysis. DNA hybridization finally identified it as Bacillus velezensis. Among the PGP attributes, the strain K-9 was found to be positive for indole acetic acid (IAA) production, phosphate solubilization, siderophore production, and nitrogen fixation. The isolate was found negative for potassium solubilization. The quantitative estimation of IAA product to 9.09 mu g/ml. The isolate also had the ability to produce lytic enzymes such as amylase and protease. The quantitative estimation of protease activity is 89.16 mu g/ml. The inoculation strain K-9 improved bioaccumulation of roots and buds and yield in the potato compared to uninoculated control plants. Conclusion: These findings give an insight into the ways to use PGP bacteria to increase potato production.