Synthesis and characteristics of a rebaudioside-A like compound as a potential non-caloric natural sweetener by Leuconostoc kimchii dextransucrase

被引：5

作者：

Kang, Hye-Jin ^{[1
]}

Lee, Ha-Nul ^{[1
]}

Hong, Seong-Jin ^{[1
]}

Park, Bo-Ram ^{[2
]}

Ameer, Kashif ^{[3
,4
]}

Cho, Jeong-Yong ^{[1
]}

Kim, Young-Min ^{[1
]}

机构：

[1] Chonnam Natl Univ, Dept Integrat Food Biosci & Biotechnol, Gwangju 61186, South Korea

[2] Natl Inst Agr Sci, Dept Agrofood Resources, Rural Dev Adm, Jeonju 55365, South Korea

[3] Univ Lahore, Univ Inst Diet & Nutr Sci, Fac Allied Hlth Sci, Lahore 54000, Pakistan

[4] Univ Sargodha, Inst Food Sci & Nutr, Sargodha 40100, Pakistan

来源：

FOOD CHEMISTRY | 2022年 / 366卷

基金：

新加坡国家研究基金会;

关键词：

Dextransucrase; Glucosyl stevioside; Stevia rebaudiana; Stevioside; Rebaudioside A; STEVIA-REBAUDIANA BERTONI; RESPONSE-SURFACE METHODOLOGY; RUBUSOSIDE DERIVATIVES; MALTASE-GLUCOAMYLASE; STEVIOSIDE; GLUCOSIDASE; STABILITY; TRANSGLUCOSYLATION; OPTIMIZATION; EXTRACTION;

D O I：

10.1016/j.foodchem.2021.130623

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Stevioside (ST) is currently considered as a highly-demanded natural and zero-caloric replacer of sucrose with several health-promoting properties. Nonetheless, its bitter aftertaste limits its use in the food industry. Herein, glucosyl steviosides were synthesized using primarily a food-grade lactic acid bacteria, Leuconostoc kimchii dextransucrase and conversion yield (%) was 40.3%. A glucose moiety was transferred stereo-selectively to ST by alpha-1,6-linkage and this is the first report about obtaining rebaudioside A (Reb-A) like glucosyl stevioside-2 (STG-2). Glucosyl steviosides revealed greatly improved stability up to 120 degrees C and remained stable over 32.1% and 58.12% in the pH (1.4) compared with 30.55% of ST. Moreover, the glucosylated steviosides improved the stability, reaching 95% after 30 days and Reb-A like compound (STG-2) especially exhibited higher stability in commercial beverages. Furthermore, the glucosyl steviosides showed over 1.92- and 2.24-fold decreases than that of enzymatically modified ST in the glucose generation rate test.

引用

页数：9