Acid gelation of reconstituted milk protein concentrate suspensions: Influence of lactose addition

Dispersions (4%, w/w, protein) of reconstituted milk protein concentrate (MPC) powders varying in protein content (35-90% protein on dry matter basis) were standardized to a lactose content that was either unadjusted, 5.6 or 11.2% (w/w) and then heated at 90 degrees C for 10 min at pH 6.7. Protein interactions in heated milk and rheological properties and microstructure of acid gels formed by the addition of glucono-delta-lactone (GDL) were studied. Heat-induced dissociation of kappa-casein was influenced by the lactose content of dispersions made from high protein MPC powders. A decrease in elastic moduli (G') of acid gels at pH 4.6 with increasing MPC protein content was offset by lactose standardization of the dispersions. An increase in gelation pH and in water holding capacity of acid gels was observed with lactose standardization of dispersions. Confocal microscopy revealed a decrease in porosity of acid gels with increasing lactose content of MPC dispersions. (C) 2016 Elsevier Ltd. All rights reserved.