Rapid and quantitative fluorescence detection of pathogenic spore-forming bacteria using a xanthene-Zn(II) complex chemosensor

被引:8
|
作者
Tiposoth, Perapon [1 ]
Khamsakhon, Somruethai [2 ]
Ketsub, Napong [2 ]
Pongtharangkul, Thunyarat [2 ]
Takashima, Ippei [3 ]
Ojida, Akio [3 ]
Hamachi, Itaru [4 ]
Wongkongkatep, Jirarut [2 ]
机构
[1] Mahidol Univ, Fac Sci, MDL, Bangkok 10400, Thailand
[2] Mahidol Univ, Fac Sci, Dept Biotechnol, Bangkok 10400, Thailand
[3] Kyushu Univ, Grad Sch Pharmaceut Sci, Higashi Ku, Fukuoka 8128582, Japan
[4] Kyoto Univ, Dept Synthet Chem & Biol Chem, Kyoto 6158510, Japan
关键词
Fluorescent assay; Chemosensor; Bacillus cereus; Bacillus thuringiensis; Pathogen; BACILLUS-SUBTILIS; NUCLEOSIDE POLYPHOSPHATES; ARTIFICIAL RECEPTORS; MASS-SPECTROMETRY; AQUEOUS-SOLUTION; CEREUS; SPORULATION; INITIATION;
D O I
10.1016/j.snb.2014.11.113
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A fluorescent detection using xanthene-based chemosensor was demonstrated for rapid detection and quantitative analysis of pathogenic spore-forming bacteria. The method is simple without the need for any complicated instrumentation and highly specific. This sensing system was applied to the fluorescence detection of a saliva sample contaminated with pathogenic spore-forming bacteria using micro-plate technique. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:606 / 612
页数:7
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