Genetic diversity analysis in traditional lowland rice (Oryza sativa L.) of Assam using RAPD and ISSR markers

被引：0
|
作者：
Bhuyan, Nipon [1 ]
Borah, Basanta K. [1 ]
Sarma, R. N. [1 ]
机构：
[1] Assam Agr Univ, Dept Agr Biotechnol, Jorhat 785013, Assam, India
来源：
CURRENT SCIENCE | 2007年 / 93卷 / 07期
关键词：
assam rice; genetic diversity; molecular markers; polymorphism information content;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Genetic variability among 24 rice genotypes from Assam was assessed employing random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat polymerase chain reaction (ISSR-PCR) using ten primers in each case. A total of 81 RAPD and 201 ISSR markers were generated with 92.5 and 98% polymorphism respectively. The average polymorphism information content (PIC) for RAPD was 0.741 and for ISSR markers it was 0.888. The Jaccard's similarity coefficient based on RAPD and ISSR analysis was 0.480 and 0.257 respectively. Cluster analysis and cophenetic correlation value based on the ISSR data discretely separated the accessions, according to farmer's classification. This communication reports genetic variability in rice from Assam using two molecular markers.
引用
收藏
页码：967 / 972
页数：6
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