Green fluorescent protein (GFP) fusion constructs in gene therapy research

被引：66

作者：

Wahlfors, J ^{[1
]}

Loimas, S ^{[1
]}

Pasanen, T ^{[1
]}

Hakkarainen, T ^{[1
]}

机构：

[1] Univ Kuopio, AI Virtanen Inst Mol Sci, Gen Transfer Technol Grp, Kuopio 70211, Finland

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2001年 / 115卷 / 01期

基金：

芬兰科学院;

关键词：

gene therapy; GFP fusion protein;

D O I：

10.1007/s004180000219

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The history of green fluorescent protein (GFP) as a marker is less than 10 years old, but it has already made a major impact on many areas of natural sciences, especially on cell biology and histochemistry. GFP can be detected in living cells without selection or staining and it can be fused to other proteins to yield fluorescent chimeras. The potential of GFP has also been recognised by gene therapy researchers and various GFP-tagged therapeutic proteins have been constructed. These chimeric proteins have been used to determine the expression level, site and time course of the therapeutic gene, or the correlation between gene transfer rate and therapeutic outcome. This review summarises the status of the applications of GFP fusions in gene therapy research.

引用

页码：59 / 65

页数：7

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