Styrene and ethylene glycol have a synergetic effect on lipid peroxidation that is better protected than repaired by CoQ10

Previous study of a group of 22 workers occupationally exposed to styrene, ethylene glycol and their mixture at a paint and lacquer industry indicated significantly elevated concentration of malondialdehyde with 4-hydroxynonenal (MDA + 4-HNE) in the blood plasma, successfully decreased with coenzyme Q(10) (CoQ(10)) supplementation. The aim of present study is to evaluate whether the exposure to styrene or/and ethylene glycol could be responsible for the increase in MDA level. The mechanism of a single solvent action and the mixture was examined, specially whether it is connected with hydroxyl radical ((OH)-O-.) generation. It was also investigated whether coenzyme Q(10) could be considered as a protective (given before the solvents) or repairing (given after the solvents) agent in oxidative stress caused by the solvents. The results indicate that ethylene glycol nor styrene increase MDA and (OH)-O-., but as a mixture give synergetic interaction, elevating MDA and (OH)-O-. concentration to a statistically significant extent. Coenzyme Q(10) at a dose of 3.0 mu g/ml only protects, but does not repair increased lipid peroxidation caused by ethylene glycol with styrene. In order to obtain both a protective:and repairing effect, a concentration of 12.0 mu g/ml CoQ is needed. (c) 2005 Elsevier Ltd. All rights reserved.