DNA MICROSATELLITE MARKERS FOR SWARTZIA GLAZIOVIANA (FABACEAE), A THREATENED SPECIES FROM THE BRAZILIAN ATLANTIC FOREST

被引：1
|
作者：
Spoladore, Janaina [1 ,2 ]
Mansano, Vidal F. [1 ,2 ]
Dias de Freitas, Luan C. [1 ]
Sebbenn, Alexandre M. [3 ]
Lemes, Maristerra R. [1 ,2 ,4 ]
机构：
[1] Inst Pesquisas Jardim Bot Rio de Janeiro, Diretoria Pesquisas, Lab Biol Mol Plantas, Rio De Janeiro, RJ, Brazil
[2] Inst Pesquisas Jardim Bot Rio de Janeiro, Escola Nacl Bot, Programa Pos Grad Bot Trop, Rio De Janeiro, RJ, Brazil
[3] Inst Pesquisas & Estudos Florestais, Sao Paulo, Brazil
[4] Inst Nacl de Pesquisas da Amazonia, Lab Genet & Biol Reprod Plantas LabGen, Manaus, Amazonas, Brazil
来源：
APPLICATIONS IN PLANT SCIENCES | 2016年 / 4卷 / 02期
关键词：
conservation; Fabaceae; population genetics; simple sequence repeat (SSR); Swartzia glazioviana; Swartzia spp;
D O I：
10.3732/apps.1500081
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Premise of the study: Development and characterization of a set of DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a naturally rare and threatened tree species, were carried out to investigate its conservation genetics. _ Methods and Results: Through an enriched genomic library procedure, 10 DNA microsatellite loci were isolated and characterized for the species. The mean expected heterozygosity was 0.776 (0.424-0.894). Cross-species amplifi cations of these loci were successfully tested for six congener taxa (S. apetala var. apetala, S. fl aemingii, S. langsdorffi i, S. macrostachya, S. myrtifolia var. elegans, and S. simplex var. continentalis). Conclusions: The 10 polymorphic microsatellite markers developed are quite informative and will provide a valuable resource to study the population and conservation genetics of S. glazioviana and other Swartzia species.
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页数：4
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