Cloning, DNA sequence analysis, and deletion of a gene encoding diacetyl-acetoin reductase from Lactococcus lactis

被引：6
|
作者：
Aungpraphapornchai, P [1 ]
Griffin, HG [1 ]
Gasson, MJ [1 ]
机构：
[1] Inst Food Res, Norwich NR4 7UA, Norfolk, England
来源：
DNA SEQUENCE | 1999年 / 10卷 / 03期
关键词：
lactic acid bacteria; flavour; diacetyl; diary products; gene cloning;
D O I：
10.3109/10425179909033942
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Diacetyl is produced by strains of lactic acid bacteria used in the dairy industry. Production of this important flavour compound could be increased by genetic manipulation of genes encoding enzymes involved in diacetyl metabolism. This paper reports the cloning and sequencing of the gene (dar) encoding diacetyl-acetoin reductase from Lactococcus lactis. Analysis of the DNA sequence of the dnr gene and surrounding area revealed the presence of a putative operon with similarity to the family of ABC transporter systems. The nar gene has been deleted from the chromosome by double cross-over homologous recombination.
引用
收藏
页码：163 / 172
页数：10
相关论文
共 50 条
  • [1] Cloning, DNA sequence, and regulation of expression of a gene encoding beta-galactosidase from Lactococcus lactis
    Griffin, HG
    MacCormick, CA
    Gasson, MJ
    DNA SEQUENCE, 1996, 6 (06): : 337 - 346
  • [2] CLONING, SEQUENCE AND EXPRESSION OF THE GENE ENCODING THE MALOLACTIC ENZYME FROM LACTOCOCCUS-LACTIS
    ANSANAY, V
    DEQUIN, S
    BLONDIN, B
    BARRE, P
    FEBS LETTERS, 1993, 332 (1-2) : 74 - 80
  • [3] CLONING AND MOLECULAR ANALYSIS OF THE DIHYDROFOLATE-REDUCTASE GENE FROM LACTOCOCCUS-LACTIS
    LESZCZYNSKA, K
    BOLHUIS, A
    LEENHOUTS, K
    VENEMA, G
    CEGLOWSKI, P
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (02) : 561 - 566
  • [4] CLONING AND SEQUENCE-ANALYSIS OF THE GENE ENCODING LACTOCOCCUS-LACTIS MALOLACTIC ENZYME - RELATIONSHIPS WITH MALIC ENZYMES
    DENAYROLLES, M
    AIGLE, M
    LONVAUDFUNEL, A
    FEMS MICROBIOLOGY LETTERS, 1994, 116 (01) : 79 - 86
  • [5] CLONING AND SEQUENCE-ANALYSIS OF THE DNAK GENE REGION OF LACTOCOCCUS-LACTIS SUBSP LACTIS
    EATON, T
    SHEARMAN, C
    GASSON, M
    JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 : 3253 - 3264
  • [6] CLONING AND CHARACTERIZATION OF UPP, A GENE ENCODING URACIL PHOSPHORIBOSYLTRANSFERASE FROM LACTOCOCCUS-LACTIS
    MARTINUSSEN, J
    HAMMER, K
    JOURNAL OF BACTERIOLOGY, 1994, 176 (21) : 6457 - 6463
  • [7] DNA sequence analysis of three Lactococcus lactis plasmids encoding phage resistance mechanisms
    Boucher, I
    Émond, É
    Parrot, M
    Moineau, S
    JOURNAL OF DAIRY SCIENCE, 2001, 84 (07) : 1610 - 1620
  • [8] CLONING, NUCLEOTIDE-SEQUENCE, AND REGULATORY ANALYSIS OF THE LACTOCOCCUS-LACTIS-DNAJ GENE
    VANASSELDONK, M
    SIMONS, A
    VISSER, H
    DEVOS, WM
    SIMONS, G
    JOURNAL OF BACTERIOLOGY, 1993, 175 (06) : 1637 - 1644
  • [9] CLONING AND DNA-SEQUENCE ANALYSIS OF AN X-PROLYL DIPEPTIDYL AMINOPEPTIDASE GENE FROM LACTOCOCCUS-LACTIS SUBSP LACTIS NCDO-763
    NARDI, M
    CHOPIN, MC
    CHOPIN, A
    CALS, MM
    GRIPON, JC
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (01) : 45 - 50
  • [10] CLONING AND DNA-SEQUENCE ANALYSIS OF A LACTOCOCCUS BACTERIOPHAGE LYSIN GENE
    SHEARMAN, C
    UNDERWOOD, H
    JURY, K
    GASSON, M
    MOLECULAR AND GENERAL GENETICS, 1989, 218 (02): : 214 - 221
12345