MEM-FLIM: all-solid-state camera for fluorescence lifetime imaging

被引：1

作者：

Zhao, Qiaole ^{[1
]}

Young, Ian Ted ^{[1
]}

Schelen, Ben ^{[1
]}

Schouten, Raymond ^{[1
]}

van den Oever, Rein ^{[1
]}

Leenen, Rene ^{[2
]}

van Kuijk, Harry ^{[2
]}

Peters, Inge ^{[2
]}

Polderdijk, Frank ^{[2
]}

Bosiers, Jan ^{[2
]}

Jalink, Kees ^{[3
]}

de Jong, Sander ^{[4
]}

van Geest, Bert ^{[4
]}

Stoop, Karel ^{[4
]}

机构：

[1] Delft Univ Technol, Delft, Netherlands

[2] Teledyne DALSA Professional Imaging, Eindhoven, Netherlands

[3] Netherlands Canc Inst, Amsterdam, Netherlands

[4] Lambert Instruments BV, Roden, Netherlands

来源：

IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES X | 2012年 / 8225卷

关键词：

Fluorescence Lifetime Imaging Microscopy (FLIM); Charge-Coupled Device (CCD); Frequency-domain FLIM;

D O I：

10.1117/12.905345

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have built an all-solid-state camera which is directly modulated at the pixel level for frequency domain fluorescence lifetime imaging microscopy (FLIM) measurement. This novel camera eliminates the need for an image intensifier through the use of an application-specific CCD design, which is being used in a frequency domain FLIM system. The first stage of evaluation for the camera has been carried out. Camera characteristics such as noise distribution, dark current influence, camera gain, sampling density, sensitivity, linearity of photometric response, and contrast modulation transfer function have been studied through experiments. We are able to do lifetime measurement using MEM-FLIM cameras for various objects, e. g. fluorescence plastic test slides, fluorescein solution, fixed GFP cells, and GFP - Actin stained live cells.

引用

页数：11

共 50 条

[1] Modulated electron-multiplied fluorescence lifetime imaging microscope: all-solid-state camera for fluorescence lifetime imaging
Zhao, Qiaole
Schelen, Ben
Schouten, Raymond
van den Oever, Rein
Leenen, Rene
van Kuijk, Harry
Peters, Inge
Polderdijk, Frank
Bosiers, Jan
Raspe, Marcel
Jalink, Kees
de Jong, Jan Geert Sander
van Geest, Bert
Stoop, Karel
Young, Ian Ted
JOURNAL OF BIOMEDICAL OPTICS, 2012, 17 (12)
[2] Fluorescence lifetime imaging using a diode-pumped all-solid-state laser system
Jones, R
Dowling, K
Cole, MJ
Parsons-Karavassilis, D
Lever, MJ
French, PMW
Hares, JD
Dymoke-Bradshaw, AKL
ELECTRONICS LETTERS, 1999, 35 (04) : 256 - 258
[3] All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing
Esposito, A
Oggier, T
Gerritsen, HC
Lustenberger, F
Wouters, FS
OPTICS EXPRESS, 2005, 13 (24): : 9812 - 9821
[4] Multiphoton fluorescence lifetime imaging microscopy (FLIM) using a streak camera
Krishna, RV
Saitoh, H
Terada, H
Centonze, VE
Herman, B
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES III, 2003, 4963 : 165 - 174
[5] Multiphoton fluorescence Lifetime Imaging Microscopy (FLIM) using a streak camera.
Herman, B
Ramanujan, K
Centonze, V
Lopez-Cruzan, M
DEVELOPMENTAL BIOLOGY, 2005, 283 (02) : 578 - 578
[6] Fluorescence lifetime imaging microscopy (FLIM)
van Munster, EB
Gadella, TWJ
MICROSCOPY TECHNIQUES, 2005, 95 : 143 - 175
[7] Lifetime imaging of luminescent oxygen sensors based on all-solid-state technology
Hartmann, P
Ziegler, W
ANALYTICAL CHEMISTRY, 1996, 68 (24) : 4512 - 4514
[8] All-solid-state ultrafast lasers facilitate multiphoton excitation fluorescence imaging
Wokosin, DL
Centonze, V
White, JG
Armstrong, D
Robertson, G
Ferguson, AI
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 1996, 2 (04) : 1051 - 1065
[9] Deconvolution in Fluorescence Lifetime imaging microscopy (FLIM)
Mannam, Varun
Yuan, Xiaotong
Howard, Scott
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XXII, 2022, 11965
[10] Fluorescence lifetime imaging using a compact, low-cost, diode-based all-solid-state regenerative amplifier
Mendez, E
Elson, DS
Koeberg, M
Dunsby, C
Bradley, DDC
French, PMW
REVIEW OF SCIENTIFIC INSTRUMENTS, 2004, 75 (05): : 1264 - 1267

← 1 2 3 4 5 →