Urinary Albumin Detected by Multi-band Multi-optical Path Length Spectroscopy Technique

To discuss the feasibility of using the visible-near infrared multi-optical path length spectroscopic technique for urinary albumin determination. This research establishes the foundation scopic information for measuring the concentration of the human urinary albumin, an automatic micro-displacement measuring device was designed, by which visible-near infrared multi-optical path length (0-4.0 mm) spectra of urine samples were obtained. The partial least square (PLS) was used to set up the models of visible, near infrared and visible-near infrared dual-band multi-optical path length absorption spectra of urine samples against the chemical value. The model in which the visible-near infrared dual-band multi-optical path length absorption spectrum used was the best of these three models. The coefficient of determination (r2) of cross-validation was 0.9905 and the most absoluteerror of prediction was 7.56 mg/L. These results prove the feasibility of the multi-optical path length for free of reagent conveniently and rapidly detecting the urinary albumin and other components.