Detection of Cryptococcus neoformans gene in patients with pulmonary cryptococcosis

被引：33

作者：

Tanaka, K ^{[1
]}

Miyazaki, T ^{[1
]}

Maesaki, S ^{[1
]}

Mitsutake, K ^{[1
]}

Kakeya, H ^{[1
]}

Yamamoto, Y ^{[1
]}

Yanagihara, K ^{[1
]}

Hossain, MA ^{[1
]}

Tashiro, T ^{[1
]}

Kohno, S ^{[1
]}

机构：

[1] NAGASAKI UNIV,SCH MED,DEPT INTERNAL MED 2,NAGASAKI 52,JAPAN

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 11期

关键词：

D O I：

10.1128/JCM.34.11.2826-2828.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pulmonary cryptococcosis was diagnosed by nested PCR. Extraction of DNA was performed by mechanical destruction of the capsules of Cryptococcus neoformans by the glass bead technique. Nested PCR was positive for 4 of 5 culture-positive specimens but negative for 1 culture-positive specimen, 10 culture-negative specimens, and 1 specimen with undetermined culture results.

引用

页码：2826 / 2828

页数：3

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