Effects of different storage conditions on aflatoxin formation in Kome inoculated with Aspergillus parasiticus NRRL 2999

In this study, Kome with walnuts were investigated for aflatoxin production. One part of stringed walnuts was inoculated at 10(4) spores/g with the Aspergillus parasiticus NRRL 2999 strain, and the other as control group was not inoculated. The Kome samples were stored at 20 +/- 1C at 85 +/- 2% and 95 +/- 2% relative humidity for 45 days. All the samples were subjected to aflatoxin B-1 (AFB(1)) and total aflatoxin analysis in certain time intervals (1, 4, 7, 15, 30 and 45 days). Enzyme-linked immunosorbent assay was used to detect the amounts of aflatoxins. At 85 +/- 2% and 95 +/- 2% relative humidity, the detected amounts of aflatoxins were 616.15 ng/kg AFB(1) and 1747.16 ng/kg aflatoxin total, and 732.98 ng/kg AFB(1) and 2039.02 ng/kg total at the end of 45 days, respectively. The findings of this research indicated that aflatoxin formation in Kome is dependent on the storage conditions, such as mold contamination, and relative humidity. Aflatoxin production was found in high levels at the 95 +/- 2% relative humidity.