Hydrolyzed collagen-grafted-poly[(acrylic acid)-co-(methacrylic acid)] hydrogel for drug delivery

Hydrolyzed collagen-grafted-poly[(acrylic acid)-co-(methacrylic acid)] hydrogels were synthesized by solution polymerization and confirmed by infrared spectroscopy. From sequential univariate analysis, the optimal molar ratio of acrylic acid: methacrylic acid was 92: 8 in the presence of N, N'-methylenebisacrylamide, ammonium persulfate, and N, N, N' N'-tetramethylethylenediamine at 0.12, 0.015, and 0.2% mol of the monomers, respectively. The water absorbency of this hydrogel was both pH-and temperaturedependent, but was higher in nonbuffered water than in boric acid/citric acid/phosphate buffer under the same conditions. The optimal hydrogel could swiftly swell and deswell in neutral and acid solutions, respectively. Its potential application in drug delivery was examined using insulin and methylene blue as model payload drugs. Loading in a 50% (v v(-1)) ethanol solution gave a higher insulin loading level than in the buffer water. Insulin and methylene blue were both released at pH 6.8 but not at pH 1.2, but followed first order kinetics and the Higuchi equation, respectively. (C) 2017 Wiley Periodicals, Inc.