Inhibitory Effects of Flavonoids from Spatholobus suberectus on Sortase A and Sortase A-Mediated Aggregation of Streptococcus mutans

被引：23

作者：

Park, Wanki ^{[1
]}

Ahn, Chan-Hong ^{[1
]}

Cho, Hyunjoo ^{[2
]}

Kim, Chang-Kwon ^{[2
]}

Shin, Jongheon ^{[2
]}

Oh, Ki-Bong ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Agr & Life Sci, Dept Agr Biotechnol, Seoul 08826, South Korea

[2] Seoul Natl Univ, Nat Prod Res Inst, Coll Pharm, Seoul 08826, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2017年 / 27卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

Spatholobus suberectus; flavonoids; Streptococcus mutans; sortase A; cell aggregation; STAPHYLOCOCCUS-AUREUS; BACTERIAL SURFACE; TRANSPEPTIDASE; PROTEINS; EXPRESSION;

D O I：

10.4014/jmb.1704.04001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Seven flavonoids were isolated from Spatholobus suberectus via repetitive column chromatography and high-performance liquid chromatography. The chemical structures of these compounds were identified by spectroscopic analysis and comparison with values reported in the literature. Among the flavonoids tested, 7-hydroxy-6-methoxyflavanone (1) and formononetin (4) exhibited strong inhibitory activity against Streptococcus mutans SrtA, with IC50 values of 46.1 and 41.8 mu M, respectively, but did not affect cell viability. The onset and magnitude of inhibition of saliva-induced aggregation in S. mutans treated with compounds 1 and 4 were comparable to the behavior of a srtA-deletion mutant without treatment.

引用

页码：1457 / 1460

页数：4

共 23 条

[1] Flavonoid Glycosides Inhibit Sortase A and Sortase A-Mediated Aggregation of Streptococcus mutans, an Oral Bacterium Responsible for Human Dental Caries
Yang, Woo-Young
Kim, Chang-Kwon
Ahn, Chan-Hong
Kim, Heegyu
Shin, Jongheon
Oh, Ki-Bong
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 26 (09) : 1566 - 1569
[2] Sortase A-mediated modification of the Streptococcus mutans transcriptome and virulence traits
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MOLECULAR ORAL MICROBIOLOGY, 2019, 34 (05) : 219 - 233
[3] Streptococcus mutans sortase A inhibitory metabolites from the flowers of Sophora japonica
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BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2015, 25 (07) : 1394 - 1397
[4] Astilbin Inhibits the Activity of Sortase A from Streptococcus mutans
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MOLECULES, 2019, 24 (03)
[5] Inhibitory Effects of Streptomyces sp. MBTH32 Metabolites on Sortase A and Sortase A-Mediated Cell Clumping of Staphylococcus aureus to Fibrinogen
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[6] Effects of missense mutations in sortase A gene on enzyme activity in Streptococcus mutans
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[7] Effects of missense mutations in sortase A gene on enzyme activity in Streptococcus mutans
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[8] 20S Proteasome Inhibitory Activity of Flavonoids isolated from Spatholobus suberectus
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[9] In-Silico Investigation on the Inhibitory Effect of Compounds from Essential Oils of Boesenbergia rotunda on Sortase-A of Streptococcus mutans
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[10] Sortase A-mediated Synthesis of Insulin Fusion Derivatives from a New Insulin Precursor
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