Experimental infection of meadow voles (Microtus pennsylvanicus) with sheep scrapie

被引:0
|
作者
Carlson, Christina M. [1 ,2 ]
Schneider, Jay R. [1 ]
Pedersen, Joel A. [2 ,3 ,4 ]
Heisey, Dennis M. [1 ]
Johnson, Christopher J. [1 ]
机构
[1] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI 53711 USA
[2] Univ Wisconsin, Program Cellular & Mol Biol, Madison, WI 53706 USA
[3] Univ Wisconsin, Dept Civil & Environm Engn, Dept Soil Sci, Madison, WI 53706 USA
[4] Univ Wisconsin, Environm Chem & Technol Program, Madison, WI 53706 USA
关键词
PROTEIN IN-VITRO; CHINESE-HAMSTERS; PRION DISEASES; STRAINS; AGENT; HOST; VIVO;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Meadow voles (Microtus pennsylvanicus) are permissive to chronic wasting disease (CWD) infection, but their susceptibility to other transmissible spongiform encephalopathies (TSEs) is poorly characterized. In this initial study, we intracerebrally challenged 6 meadow voles with 2 isolates of sheep scrapie. Three meadow voles acquired a TSE after the scrapie challenge and an extended incubation period. The glycoform profile of proteinase K-resistant prion protein (PrPres) in scrapie-sick voles remained similar to the sheep inocula, but differed from that of voles clinically affected by CWD. Vacuolization patterns and disease-associated prion protein (PrPSc) deposition were generally similar in all scrapie-affected voles, except in the hippocampus, where PrPSc staining varied markedly among the animals. Our results demonstrate that meadow voles can acquire a TSE after intracerebral scrapie challenge and that this species could therefore prove useful for characterizing scrapie isolates.
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页码:68 / 73
页数:6
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