Genotyping (AFLP) of Campylobacter spp. in recurring Campylobacter positive broiler flocks

Investigations using the tool of Amplified Fragment Length Polymorphism (AFLP) analysis could show that some broiler flocks were colonised with AFLIP genotypes of a single cluster. However, the majority of broiler flocks harboured AFLP genotypes of different clusters, with one dominant cluster. Additionally, some flocks of recurring Compylobocter positive broiler flocks of the same stable harboured AFLP genotypes of identical clusters. Dominant and reiterating genotypes demonstrate the presence of single and persistent sources of Campylobacter spp. in the environment. This may facilitate traceability to the origin and makes it possible to control contamination at the farm level. Colonisation with sporadic isolates was also found, but control of several colonisation sources would be more difficult.