A New ICEclc Subfamily Integrative and Conjugative Element Responsible for Horizontal Transfer of Biphenyl and Salicylic Acid Catabolic Pathway in the PCB-Degrading Strain Pseudomonas stutzeri KF716

被引：8

作者：

Hirose, Jun ^{[1
]}

Watanabe, Takahito ^{[2
]}

Futagami, Taiki ^{[3
]}

Fujihara, Hidehiko ^{[4
]}

Kimura, Nobutada ^{[5
]}

Suenaga, Hikaru ^{[6
]}

Goto, Masatoshi ^{[7
]}

Suyama, Akiko ^{[4
]}

Furukawa, Kensuke ^{[4
]}

机构：

[1] Univ Miyazaki, Fac Engn, Dept Appl Chem, Miyazaki 8892192, Japan

[2] Kyoto Univ, Res Inst Sustainable Humanosphere, Uji 6110011, Japan

[3] Kagoshima Univ, Fac Agr, Educ & Res Ctr Fermentat Studies, Kagoshima 8900065, Japan

[4] Beppu Univ, Fac Food & Nutr Sci, Dept Food & Fermentat Sci, Beppu, Oita 8748501, Japan

[5] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki 3058566, Japan

[6] Natl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst, Tokyo 1350064, Japan

[7] Saga Univ, Fac Agr, Saga 8408502, Japan

来源：

MICROORGANISMS | 2021年 / 9卷 / 12期

关键词：

horizontal gene transfer; ICEbph-sal; ICEclc; ICEXTD; integrative and conjugative elements; polychlorinated biphenyls; Pseudomonas stutzeri; salicylic acid; COMPLETE GENOME SEQUENCE; DEGRADATION; ISLANDS; GENES; B13;

D O I：

10.3390/microorganisms9122462

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Integrative and conjugative elements (ICEs) are chromosomally integrated self-transmissible mobile genetic elements. Although some ICEs are known to carry genes for the degradation of aromatic compounds, information on their genetic features is limited. We identified a new member of the ICEclc family carrying biphenyl catabolic bph genes and salicylic acid catabolic sal genes from the PCB-degrading strain Pseudomonas stutzeri KF716. The 117-kb ICE(bph-sal)KF716 contains common core regions exhibiting homology with those of degradative ICEclc from P. knackmussii B13 and ICEXTD from Azoarcus sp. CIB. A comparison of the gene loci collected from the public database revealed that several putative ICEs from P. putida B6-2, P, alcaliphila JAB1, P. stutzeri AN10, and P. stutzeri 2A20 had highly conserved core regions with those of ICE(bph-sal)KF716, along with the variable region that encodes the catabolic genes for biphenyl, naphthalene, toluene, or phenol. These data indicate that this type of ICE subfamily is ubiquitously distributed within aromatic compound-degrading bacteria. ICE(bph-sal)KF716 was transferred from P. stutzeri KF716 to P. aeruginosa PAO1 via a circular extrachromosomal intermediate form. In this study, we describe the structure and genetic features of ICE(bph-sal)KF716 compared to other catabolic ICEs.

引用

页数：11