Early Detection and Identification of the Main Fungal Pathogens for Resistance Evaluation of New Genotypes of Forest Trees

被引：5
|
作者：
Shestibratov, Konstantin A. [1 ,2 ]
Baranov, Oleg Yu. [3 ]
Subbotina, Natalya M. [1 ,2 ]
Lebedev, Vadim G. [1 ,2 ]
Panteleev, Stanislav V. [3 ]
Krutovsky, Konstantin V. [4 ,5 ,6 ,7 ]
Padutov, Vladimir E. [3 ]
机构：
[1] Russian Acad Sci, Forest Biotechnol Grp, Branch Shemyakin, 6 Prospect Nauki, Moscow 142290, Russia
[2] Russian Acad Sci, Ovchinnikov Inst Bioorgan Chem, 6 Prospect Nauki, Moscow 142290, Russia
[3] Natl Acad Sci Belarus, Forest Res Inst, Lab Genet & Biotechnol, 71 Proletarskaya Str, Gomel 246001, BELARUS
[4] George August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgenweg 2, D-37077 Gottingen, Germany
[5] Russian Acad Sci, Vavilov Inst Gen Genet, Lab Populat Genet, Gubkina Str 3, Moscow 119991, Russia
[6] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Genome Res & Educ Ctr, Lab Forest Gen, Akademgorodok 50a-2, Krasnoyarsk 660036, Russia
[7] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA
来源：
FORESTS | 2018年 / 9卷 / 12期
关键词：
Betula; birch; fungal phytopathogens; ITS; PCR; AGENT;
D O I：
10.3390/f9120732
中图分类号：
S7 [林业];
学科分类号：
0829 ; 0907 ;
摘要：
The growing importance of forest plantations increases the demand for phytopathogen resistant forest trees. This study describes an effective method for early detection and identification of the main fungal phytopathogens in planting material of silver birch (Betula pendula) and downy birch (B. pubescens), based on the estimation of the size of the internal transcribed spacers (ITS1 and ITS2) in the 18S-5.8S-28S rDNA gene cluster, which are species-specific for most micromycetes. The electrophoretic assay of the ITS1 and ITS2 loci has allowed us to identify predominant phytopathogenic fungal species in downy and silver birch in planta. This new molecular genetic method can be used to screen birch and other forest trees for different fungal pathogens to evaluate disease resistance. This information can be useful in breeding new genotypes of forest trees, including transgenic clones with modified wood composition.
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页数：9
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