Molecular Cloning and Expression Analysis of a Delta 6-Fatty Acid Desaturase Gene from Rhizopus stolonifer Strain YF6 Which Can Accumulate High Levels of Gamma-Linolenic Acid

被引：10
|
作者：
Wan, Xia [1 ,2 ]
Zhang, Yinbo [1 ]
Wang, Ping [1 ]
Jiang, Mulan [1 ,2 ]
机构：
[1] Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China
[2] Minist Agr, Key Lab Oil Crop Biol, Wuhan 430062, Peoples R China
来源：
JOURNAL OF MICROBIOLOGY | 2011年 / 49卷 / 01期
基金：
中国国家自然科学基金;
关键词：
desaturase; Rhizopus stolonifer; gamma-linolenic acid; FATTY-ACID; CUNNINGHAMELLA-ECHINULATA; HETEROLOGOUS EXPRESSION; MORTIERELLA FUNGUS; DELTA(6)-DESATURASE; IDENTIFICATION; METABOLISM; HEALTH;
D O I：
10.1007/s12275-011-0254-7
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The delta 6-desaturase gene was cloned from Rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (GLA, C18:3 Delta(6,9,12)) to the total fatty acids. The cloned DNA contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and HPGG motif. Notably, this deduced sequence had a shorter C-terminus. Results demonstrated that the cDNA sequence exhibited delta 6-desaturase activity by accumulation of about 22.4% of GLA to the total fatty acids in the recombinant Pichia pastoris strain GS115.
引用
收藏
页码：151 / 154
页数：4
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