TaRAR1 is Required for Lr24-Mediated Wheat Leaf Rust Resistance

被引：3
|
作者：
Zhang Li-rong [1 ]
Yang Wen-xiang [1 ]
Liu Da-qun [1 ]
机构：
[1] Agr Univ Hebei, Coll Plant Protect, Biol Control Ctr Plant Dis & Pests Hebei Prov, Baoding 071001, Peoples R China
来源：
AGRICULTURAL SCIENCES IN CHINA | 2011年 / 10卷 / 11期
基金：
中国国家自然科学基金;
关键词：
disease resistance; barley stripe mosaic virus (BSMV); virus-induced gene silencing (VIGS); Lr24; RAR1; POWDERY MILDEW RESISTANCE; PLANT DEFENSE RESPONSES; TRITICUM-AESTIVUM L; STRIPE-MOSAIC-VIRUS; DISEASE RESISTANCE; HEXAPLOID WHEAT; BREAD WHEAT; LRR PROTEIN; BARLEY; ARABIDOPSIS;
D O I：
10.1016/S1671-2927(11)60173-9
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Virus-induced gene silencing (VIGS) offers a rapid and high throughout technology platform for the analysis of gene function in plants. The barley stripe mosaic virus (BSMV) VIGS system was optimized in studies silencing phytoene desaturase expression in wheat, and demonstrated that infection with BSMV construct carrying a 412 bp fragment of TaRAR1 caused conversion of incompatible to compatible interactions to Lr24-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring Lr24 whereas infection with a control construct had no effect on resistance or susceptibility. RT-PCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels. These studies indicated that TaRAR1 was a required component for Lr24-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.
引用
收藏
页码：1732 / 1738
页数：7
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