Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches

被引：78
|
作者：
Brown, J
Brown, T
Fox, KR
机构：
[1] Univ Southampton, Sch Biol Sci, Div Biochem & Mol Biol, Southampton SO16 7PX, Hants, England
[2] Univ Southampton, Dept Chem, Southampton SO16 5NH, Hants, England
来源：
BIOCHEMICAL JOURNAL | 2001年 / 354卷
关键词：
DNA mismatches; DNA recognition; mismatch repair;
D O I：
10.1042/0264-6021:3540627
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have used bandshift analysis to measure the interaction between the Escherichia coli mismatch-binding protein MutS and synthetic DNA fragments containing all possible DNA mismatches as well as an unpaired T (DeltaT). The order of affinity is found to be DeltaT > GT > GG > AA approximate to TT approximate to TC > CA > GA > CC > GC, We find that the affinity for GT mismatches is affected by the flanking sequence and decreases in the order G(n)C(n) > (GC)(n) > A(n)T(n) > (AT)(n). Studies with base analogues show good binding to phiT (where phi represents 1',2'-dideoxy-ribose), but much weaker binding to G phi.
引用
收藏
页码：627 / 633
页数：7
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