Lac operator repeats generate a traceable fragile site in mammalian cells

被引：37

作者：

Jacome, Ariana ^{[1
]}

Fernandez-Capetillo, Oscar ^{[1
]}

机构：

[1] Spanish Natl Canc Res Ctr CNIO, Genom Instabil Grp, Madrid 28029, Spain

来源：

EMBO REPORTS | 2011年 / 12卷 / 10期

基金：

欧洲研究理事会;

关键词：

anaphase bridge; PICH; common fragile site; lac operon; micronucleus; DNA-DAMAGE RESPONSE; HUMAN-CHROMOSOMES; ANAPHASE BRIDGES; REPLICATION; LESIONS; STRESS; CANCER; TRANSCRIPTION; LOCALIZATION; ACTIVATION;

D O I：

10.1038/embor.2011.158

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One limitation for the study of chromosomal fragile sites is that they must be studied on metaphase spreads, after the breakage. We show here that bacterial lac operator (lacO) repeats are prone to spontaneous breakage, which when combined with a fluorescent lac repressor (lacR) has allowed us to track a fragile site through the cell cycle. By using this system, we show that Plk1-interacting checkpoint helicase (PICH) is already present at fragile sites during interphase, suggesting roles for this helicase beyond mitosis. In addition, we report that the oncogene Myc promotes the formation of anaphase bridges and micronuclei containing fragile-site sequences.

引用

页码：1032 / 1038

页数：7

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