GFP-FRET detects swings of the lever-arm of myosin motor

被引：0
|
作者：
Sutoh, K [1 ]
机构：
[1] Univ Tokyo, Dept Life Sci, Tokyo 1538902, Japan
来源：
NA/K-ATPASE AND RELATED ATPASES | 2000年 / 1207卷
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The "lever-arm" model of a myosin motor predicts that the lever-arm domain in the myosin head tilts and swings against the catalytic domain during ATP hydrolysis, resulting in force generation. This paper describes the experiments of the fluorescence resonance energy transfer (FRET) between two fluorescent proteins (GFP and BFP) fused to the N- and C-termini of the Dictyostelium myosin motor domain to detect such swings of the lever-arm during the ATP hydrolysis cycle.
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页码：65 / 70
页数：6
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  • [1] Elastic lever-arm model for myosin V
    Vilfan, A
    [J]. BIOPHYSICAL JOURNAL, 2005, 88 (06) : 3792 - 3805
  • [2] Elastic lever-arm model for myosin V
    不详
    [J]. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2005, 34 (06): : 747 - 747
  • [3] Dynamics of the Lever-Arm Swing in Myosin V
    Trivedi, Darshan V.
    Davis, Jonathan P.
    Yengo, Christopher M.
    [J]. BIOPHYSICAL JOURNAL, 2014, 106 (02) : 178A - 178A
  • [4] The motor domain, not the lever-arm/converter domain, determines the direction of myosin movement
    Homma, K
    Yoshimura, M
    Saito, J
    Ikebe, R
    Ikebe, M
    [J]. JOURNAL OF GENERAL PHYSIOLOGY, 2001, 118 (01): : 10A - 11A
  • [5] The motor domain but not a junction of the lever-arm converter domain determines the direction of myosin movement
    Homma, K
    Yoshimura, M
    Saito, J
    Ikebe, R
    Ikebe, M
    [J]. BIOPHYSICAL JOURNAL, 2001, 80 (01) : 573A - 573A
  • [6] GFP-Tagged regulatory light chain monitors single myosin lever-arm orientation in a muscle fiber
    Burghardt, Thomas P.
    Ajtai, Katalin
    Chan, Daniel K.
    Halstead, Miriam F.
    Li, Jinhui
    Zheng, Ye
    [J]. BIOPHYSICAL JOURNAL, 2007, 93 (06) : 2226 - 2239
  • [7] Single Myosin Cross-Bridge Orientation in Cardiac Papillary Muscle Detects Lever-Arm Shear Strain in Transduction
    Burghardt, Thomas P.
    Josephson, Matthew P.
    Ajtai, Katalin
    [J]. BIOCHEMISTRY, 2011, 50 (36) : 7809 - 7821
  • [8] The azimuthal path of myosin V and its dependence on lever-arm length
    Lewis, John H.
    Beausang, John F.
    Sweeney, H. Lee
    Goldman, Yale E.
    [J]. JOURNAL OF GENERAL PHYSIOLOGY, 2012, 139 (02): : 101 - 120
  • [9] Detection of the swings of the lever arm of a myosin motor by fluorescence resonance energy transfer of green and blue fluorescent proteins
    Suzuki, Y
    [J]. METHODS, 2000, 22 (04) : 355 - 363
  • [10] Regulatory Light Chain Mutants Linked to Heart Disease affect the Cardiac Myosin Lever-Arm
    Sikkink, Laura A.
    Burghardt, Thomas P.
    [J]. BIOPHYSICAL JOURNAL, 2013, 104 (02) : 156A - 156A
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