Cell-Free Technologies for Proteomics and Protein Engineering

被引：1

作者：

Ohashi, Hiroyuki ^{[1
]}

Miyamoto-Sato, Etsuko ^{[1
,2
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Div Interactome Med Sci, Minato Ku, 4-6-1 Shirokanedai, Tokyo 1088639, Japan

[2] Tokyo Univ Sci, Div Mol Biol, Res Inst Biomed Sci, 2669 Yamazaki, Noda, Chiba 2780022, Japan

来源：

PROTEIN AND PEPTIDE LETTERS | 2016年 / 23卷 / 09期

关键词：

Cell-free; IVV (in vitro virus); mRNA display; PPI (protein-protein interaction); Proteomics; PURE system; FREE EXPRESSION SYSTEMS; IN-VITRO SELECTION; FREE TRANSLATION SYSTEM; MESSENGER-RNA DISPLAY; HIGH-THROUGHPUT ASSAY; VIRUS-LIKE PARTICLES; MEMBRANE-PROTEINS; RIBOSOME DISPLAY; ESCHERICHIA-COLI; PEPTIDE LIBRARIES;

D O I：

10.2174/0929866523666160613200304

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell-free translation systems facilitate rapid production of specific proteins and are particularly suited as high-throughput methods for whole-genome protein synthesis. Moreover, these systems do not rely on living cells, thereby allowing the synthesis of unstable or cytotoxic proteins in vitro. In this review, we describe the principles and potential applications of cell-free protein translation systems and the future prospects of proteomics approaches using next-generation sequencing and cell-free expression technologies.

引用

页码：819 / 827

页数：9

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