Different CO2 settings (6.0% vs 7.0%) do have an impact on extracellular pH of culture medium (pHe) and euploidy rates rather than on blastocyst development: a sibling oocyte study

被引:2
|
作者
Abdala, Andrea [1 ]
Elkhatib, Ibrahim [1 ]
Bayram, Asina [1 ]
Arnanz, Ana [1 ]
El-Damen, Ahmed [1 ]
Melado, Laura [1 ]
Lawrenz, Barbara [1 ,2 ]
Garrido, Nicolas [3 ]
Fatemi, Human M. [1 ]
De Munck, Neelke [1 ]
机构
[1] ART Fertil Clin, Abu Dhabi, U Arab Emirates
[2] Womens Univ Hosp Tubingen, Obstet Dept, Tubingen, Germany
[3] IVI Fdn, Inst Invest Sanitaria La Fe IIS La Fe, Valencia, Spain
关键词
Continuous culture media; Euploidy rates; TE biopsy; PGT-A; CO2; pH; INTRACELLULAR PH; EMBRYO CULTURE; CARBON-DIOXIDE; CONSEQUENCES; ENVIRONMENT; FIXATION; IVF;
D O I
10.1007/s10815-021-02303-0
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Objective To determine whether euploidy rates and blastocyst development differ in a continuous culture medium under different CO2 concentrations. Design and method A single-center retrospective study was performed from July 2018 to October 2019 including 44 fresh cycles with at least four fresh mature oocytes (MII) without severe male factor infertility. Sibling MII were injected and cultured in Global (R) Total (R) LP under 6.0% (pHe = 7.374 +/- 0.014) or 7.0% (pHe = 7.300 +/- 0.013) CO2, 5.0% O-2, and 89.0% or 88.0% N-2. Analyzed variables were normally fertilized oocytes (2PN), cleavage rate, blastulation rate on day 5/2PN, usable blastocyst (blastocysts biopsied/2PN), and euploidy rates. Blastocyst's trophectoderm biopsy was performed on day 5, 6, or 7 for genetic testing and mitochondrial DNA (mtDNA) quantification by next-generation sequencing. Results Women's mean age was 33.0 +/- 6.6 years old. From a total of 604 MII, no differences were found in normal fertilization and cleavage rates on day 3 between 6.0 and 7.0% CO2 (72.3% vs 67.1%, p = 0.169 and 96.6% vs 96.3%, p = 0.897, respectively). Blastulation rate on day 5/2PN was comparable between 6.0 and 7.0% CO2 (68.1% vs 64.2%, p = 0.409). Although usable blastocyst rate was not different (54.3% vs 55.3%, p = 0.922), total euploidy rates differed significantly (58.7% vs 42.8%, p = 0.016) between 6.0% and 7.0% CO2, respectively. The mean blastocyst mtDNA content was significantly lower in 6.0% CO2 (30.4 +/- 9.1 vs 32.9 +/- 10.3, p = 0.037). Conclusion Blastocyst development is not affected when embryos are cultured in vitro at 6.0% or 7.0% CO2, while euploidy rates are significantly decreased at a higher CO2 concentration, therefore at a lower pHe.
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页码:2915 / 2923
页数:9
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  • [1] Different CO2 settings (6.0% vs 7.0%) do have an impact on extracellular pH of culture medium (pHe) and euploidy rates rather than on blastocyst development: a sibling oocyte study
    Andrea Abdala
    Ibrahim Elkhatib
    Aşina Bayram
    Ana Arnanz
    Ahmed El-Damen
    Laura Melado
    Barbara Lawrenz
    Nicolás Garrido
    Human M. Fatemi
    Neelke De Munck
    [J]. Journal of Assisted Reproduction and Genetics, 2021, 38 : 2915 - 2923