Pulsatile stretch stimulates vascular endothelial growth factor (VEGF) secretion by cultured rat cardiac myocytes

被引:116
|
作者
Seko, Y
Seko, Y
Takahashi, N
Shibuya, M
Yazaki, Y
机构
[1] Univ Tokyo, Grad Sch Med, Dept Cardiovasc Med, Bunkyo Ku, Tokyo, Japan
[2] Juntendo Univ, Sch Med, Dept Immunol, Bunkyo Ku, Tokyo, Japan
[3] Asahi Life Fdn, Inst Adult Dis, Shinjuku Ku, Tokyo, Japan
[4] Tokyo Med & Dent Univ, Sch Med, Dept Ophthalmol, Bunkyo Ku, Tokyo 113, Japan
[5] Univ Tokyo, Inst Med Sci, Dept Genet, Minato Ku, Tokyo, Japan
关键词
D O I
10.1006/bbrc.1998.9969
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Evidence has accumulated that vascular endothelial growth factor (VEGF) is expressed in the heart, and its expression is markedly increased in response to hypoxia, Recently, it was shown that pulsatile myocardial stretch in vivo markedly enhanced VEGF mRNA level in the heart. To investigate whether pulsatile mechanical stretch really stimulates VEGF expression by cardiac myocytes, using an in vitro preparation, we examined the secretion of VEGF into the culture media from cardiac myocytes subjected to pulsatile stretch. We found that pulsatile mechanical stretch induced rapid secretion of VEGF by cultured rat cardiac myocytes and mRNA expression of VEGF and VEGF receptors in the cardiac myocytes, We also found that the stretch-induced secretion of VEGF was at least in part mediated by TGF-beta. These data provide the direct evidence that mechanical overload itself can induce VEGF secretion by cardiac myocytes, which may play a role in ameliorating the relative myocardial hypoxia, (C) 1999 Academic Press.
引用
收藏
页码:462 / 465
页数:4
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