DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs

被引：10

作者：

Liu, Chen ^{[1
]}

Han, Jialun ^{[1
]}

Zhou, Lujian ^{[1
]}

Zhang, Jingjing ^{[1
]}

Du, Jie ^{[1
]}

机构：

[1] Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Coll Mat Sci & Engn, Haikou 570228, Hainan, Peoples R China

来源：

SENSORS | 2020年 / 20卷 / 07期

基金：

中国国家自然科学基金; 海南省自然科学基金;

关键词：

microRNAs; deoxyribozyme; rolling-circle amplification; signal amplification; GRAPHENE OXIDE; DNA MACHINE; IN-SITU;

D O I：

10.3390/s20072017

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L-1 level hence affording a simple, highly sensitive, and selective low cost detection platform.

引用

页数：12

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