Experimental teaching is a very important part of the cultivation of application-oriented talent, so the evaluation of experimental teaching quality is the key to measure the effectiveness of the cultivation. This paper through the analysis of application-oriented experimental teaching system, tries to establish a set of classified experimental teaching quality evaluation system, then uses fuzzy comprehensive evaluation techniques to strengthen the evaluation system of scientific and effective, striving to make the evaluation system focusing on both process and results, and make the students and teachers feel encouraging and developing.