Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

被引：2
|
作者：
Wu, Jheng-Syong [1 ,2 ]
Chung, Yung-Chin [1 ]
Chien, Jun-Jei [1 ]
Chou, Chien [1 ,3 ]
机构：
[1] Chang Gung Univ, Grad Inst Electopt Engn, Taoyuan, Taiwan
[2] Acad Sinica, Inst Chem, Taipei, Taiwan
[3] Chang Gung Univ, Res Ctr Biomed Engn, Taoyuan, Taiwan
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2018年 / 23卷 / 01期
关键词：
confocal fluorescence microscopy; aberrations; optical heterodyne; ZEEMAN LASER; SPHERICAL-ABERRATION; SCATTERING MEDIUM; TURBID MEDIA; REDUCTION;
D O I：
10.1117/1.JBO.23.1.010502
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed. (c) 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
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页数：4
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