Label-free and amplified detection of apoptosis-associated caspase activity using branched rolling circle amplification

被引：13

作者：

Liu, Meng ^{[1
]}

Zhang, Di ^{[1
]}

Zhang, Xuechong ^{[2
]}

Xu, Qinfeng ^{[2
]}

Ma, Fei ^{[1
]}

Zhang, Chun-yang ^{[1
]}

机构：

[1] Shandong Normal Univ, Shandong Prov Key Lab Clean Prod Fine Chem, Collaborat Innovat Ctr Functionalized Probes Chem, Minist Educ,Coll Chem Chem Engn & Mat Sci,Key Lab, Jinan 250014, Peoples R China

[2] Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 39期

基金：

中国国家自然科学基金;

关键词：

ACTIVITY ASSAY; FLUORESCENCE; ACTIVATION; PROBE; TELOMERASE; TOOL;

D O I：

10.1039/d0cc01564d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We develop a label-free fluorescence method for ultrasensitive detection of apoptosis-associated caspase activity based on branched rolling circle amplification. This work demonstrates for the first time the use of a nucleic acid amplification strategy for the amplified detection of caspase activity, which allows for highly sensitive detection of endogenous caspase even at the single-cell level. This method can be further applied for kinetic analysis and inhibitor screening.

引用

页码：5243 / 5246

页数：4

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