Large-scale solution synthesis of phosphorothioate oligonucleotides: A comparison of the phosphoroamidite and phosphotriester dimeric approaches

被引：0

作者：

Bonora, GM

Zaramella, S

Ravikumar, V

机构：

[1] Univ Trieste, Dept Chem Sci, Trieste, Italy

[2] Karolinska Inst, MBB, Stockholm, Sweden

[3] ISIS Pharmaceut, Carlsbad, CA 92008 USA

来源：

CROATICA CHEMICA ACTA | 2001年 / 74卷 / 04期

关键词：

oligonucleotides; phosphorothioate; scale up; large-scale; synthesis; phosphoramidite; phosphotriester; liquid-phase; polyethylene glycol;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two different approaches to the solution synthesis of oligonucleotides are compared. Both procedures use dimer synthons as building blocks to save time and to improve the final product yield and quality. These strategies look quite promising due to their easy upscaling, providing an advantageous alternative to the well-established solid-phase methods.

引用

页码：779 / 786

页数：8

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