SINGLE-MOLECULE DUAL-BEAM OPTICAL TRAP ANALYSIS OF PROTEIN STRUCTURE AND FUNCTION

被引：23

作者：

Sung, Jongmin ^{[1
,2
]}

Sivaramakrishnan, Sivaraj ^{[1
]}

Dunn, Alexander R. ^{[3
]}

Spudich, James A. ^{[1
]}

机构：

[1] Stanford Univ, Dept Biochem, Sch Med, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Appl Phys, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA

来源：

METHODS IN ENZYMOLOGY, VOL 475: SINGLE MOLECULE TOOLS, PT B: SUPER-RESOLUTION, PARTICLE TRACKING, MULTIPARAMETER, AND FORCE BASED METHODS | 2010年 / 475卷

关键词：

MYOSIN-V; SLIDING MOVEMENT; ACTIN-FILAMENTS; CARDIAC MYOSIN; SMOOTH-MUSCLE; POWER STROKE; FORCE; RESOLUTION; MOTOR; CONSTRUCTION;

D O I：

10.1016/S0076-6879(10)75014-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optical trapping is one of the most powerful single-molecule techniques. We provide a practical guide to set up and use an optical trap, applied to the molecular motor myosin as an example. We focus primarily on studies of myosin function using a dual-beam optical trap, a protocol to build such a trap, and the experimental and data analysis protocols to utilize it.

引用

页码：321 / 375

页数：55

共 50 条

[1] Trapping positions in a dual-beam optical trap
Rafferty, Aidan
Preston, Thomas C.
JOURNAL OF APPLIED PHYSICS, 2021, 130 (18)
[2] Chiral particles in the dual-beam optical trap
Brzobohaty, Oto
Hernandez, Raul Josue
Simpson, Stephen
Mazzulla, Alfredo
Cipparrone, Gabriella
Zemanek, Pavel
OPTICS EXPRESS, 2016, 24 (23): : 26382 - 26391
[3] Optohydrodynamic theory of particles in a dual-beam optical trap
Gordon, R.
Kawano, M.
Blakely, J. T.
Sinton, D.
PHYSICAL REVIEW B, 2008, 77 (24):
[4] Single-molecule analysis of telomerase structure and function
Hengesbach, Martin
Akiyama, Benjamin M.
Stone, Michael D.
CURRENT OPINION IN CHEMICAL BIOLOGY, 2011, 15 (06) : 845 - 852
[5] Advances in Laser Dual-Trap Optical Tweezers in Single-Molecule Biophysics
Fan, Gong
Ren, Yuxuan
CHINESE JOURNAL OF LASERS-ZHONGGUO JIGUANG, 2023, 50 (15):
[6] The lensing effect of trapped particles in a dual-beam optical trap
Grosser, Steffen
Fritsch, Anatol W.
Kiessling, Tobias R.
Stange, Roland
Kaes, Josef A.
OPTICS EXPRESS, 2015, 23 (04): : 5221 - 5235
[7] Temperature-Controlled Dual-Beam Optical Trap for Single Particle Studies of Organic Aerosol
Logozzo, Alexander
Preston, Thomas C.
JOURNAL OF PHYSICAL CHEMISTRY A, 2022, 126 (01): : 109 - 118
[8] Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap
Solmaz, Mehmet E.
Biswas, Roshni
Sankhagowit, Shalene
Thompson, James R.
Mejia, Camilo A.
Malmstadt, Noah
Povinelli, Michelle L.
BIOMEDICAL OPTICS EXPRESS, 2012, 3 (10): : 2419 - 2427
[9] Optical manipulation of anisotropic spherical shell particles in a dual-beam trap
Bai, Jing
Gao, Yu
Ge, Cheng-Xian
Wu, Zhen-Sen
Xiao, Chang
PHYSICA SCRIPTA, 2024, 99 (03)
[10] Optical manipulation of airborne particles using flexible dual-beam trap
Brzobohaty, Oto
Siler, Martin
Zemanek, Pavel
OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION IX, 2012, 8458

← 1 2 3 4 5 →