Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli

被引：15
|
作者：
Wang, Xiangwei [1 ,2 ]
Li, Lingling [1 ,3 ]
Zheng, Yanning [1 ,2 ]
Zou, Huibin [1 ]
Cao, Yujin [1 ]
Liu, Hui [1 ]
Liu, Wei [1 ]
Xian, Mo [1 ]
机构：
[1] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
[3] Sichuan Agr Univ, Coll Food Sci, Yaan 625014, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2012年 / 114卷
基金：
美国国家科学基金会;
关键词：
Long chain hydroxyfatty acid; Escherichia coli; Thioesterase; Fatty acid hydroxylase; fadD knocking out; FATTY-ACIDS; BACILLUS-MEGATERIUM; MICROBIAL-PRODUCTION; OLEIC-ACID; MONOOXYGENASE; HYDROXYLATION; OMEGA; BIOCATALYSIS; THIOESTERASE; RECOMBINANT;
D O I：
10.1016/j.biortech.2012.02.119
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
This study devised a pathway in Escherichia coli for direct production of long chain hydroxyfatty acids (HFAs) from glucose. This is first report on the biosynthesis of HFAs from renewable sugar, without the need of exogenous fatty acids. By employing thioesterases BTE and 'TesA to tailor the composition of free fatty acids (FFAs) and using fatty acid hydroxylase P450(BM3) to convert FFAs to HFAs, high-specificity production of C12 and C14 HFAs was achieved. By further knocking out the endogenous fadD gene of E. coli, an engineered strain capable of producing 117.0 mg/L HFAs was finally obtained, representing a high HFA production in shake flask. This study indicated an attractive metabolic strategy for the biosynthesis of HFAs directly from renewable carbohydrates resources. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：561 / 566
页数：6
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