Protein fractionation of pH-responsive brush-modified ethylene vinyl alcohol copolymer membranes

被引：7

作者：

Ye, Hui ^{[1
]}

Zhou, Yining ^{[1
]}

Yang, Guodong ^{[1
]}

Yu, Tengfei ^{[1
]}

Zhang, Yuzhong ^{[1
]}

Zhao, Lizhi ^{[1
]}

Xin, Qingping ^{[1
]}

Han, Shurui ^{[1
]}

机构：

[1] Tiangong Univ, Sch Mat Sci & Engn, State Key Lab Separat Membranes & Membrane Proc, Tianjin 300387, Peoples R China

来源：

POLYMER ENGINEERING AND SCIENCE | 2022年 / 62卷 / 04期

基金：

中国国家自然科学基金;

关键词：

atom transfer radical polymerization (ATRP); membranes; proteins; separation techniques; POLYMERIZATION; SEPARATION;

D O I：

10.1002/pen.25904

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Protein fractionation and purification remain challenging in membrane separation. Herein, a pH-responsive membrane was developed by grafting the brush-like poly(dimethylaminoethyl methacrylate) (PDMAEMA) via ATRP onto the ethylene vinyl alcohol copolymer (EVAL) membrane for protein fractionation. The PDMAEMA brushes are distributed in all pore channels of the membrane. Based on the electrostatic and sieving properties of the as-prepared pH-responsive membrane, three milk proteins are successfully separated from their mixture. The alpha-Lactalbumin (alpha-LA) preferentially permeates the membrane from the mixture at pH 3.2, and the bovine serum albumin (BSA) and lactoferrin (LF) can be separated at pH 6.4 based on the selective adsorption. This work proposes a promising candidate for protein separation by a pH-responsive membrane.

引用

页码：1041 / 1051

页数：11

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