Novel additive for sperm cryopreservation media: Holotheria parva coelomic cavity extract protects human spermatozoa against oxidative stress-A pilot study

被引：3

作者：

Khashavi, Zahra ^{[1
]}

Homaei, Ahmad ^{[2
]}

Koohnavard, Fahimeh ^{[3
]}

Kamrani, Ehsan ^{[2
,4
]}

Spinaci, Marcella ^{[5
]}

Luwor, Rodney B. ^{[6
]}

Archang, Mahsa ^{[3
]}

Agarwal, Ashok ^{[7
]}

Henkel, Ralf ^{[7
,8
]}

机构：

[1] Infertil Therapy & IVF Ctr Om E Leila Hosp, Bandar Abbas, Iran

[2] Univ Hormozgan, Fac Marine Sci & Technol, Dept Marine Biol, POB 3995, Bandar Abbas, Iran

[3] Hormozgan Univ Med Sci, Bandar Abbas, Iran

[4] Univ Hormozgan, Fac Marine Sci & Technol, Dept Fisheries Sci, Bandar Abbas, Iran

[5] Univ Bologna, Dept Vet Med Sci, Bologna, Italy

[6] Univ Melbourne, Royal Melbourne Hosp, Dept Surg, Parkville, Vic, Australia

[7] Cleveland Clin, Amer Ctr Reprod Med, Cleveland, OH 44106 USA

[8] Univ Western Cape, Dept Med Biosci, Cape Town, South Africa

来源：

ANDROLOGIA | 2020年 / 52卷 / 06期

关键词：

cryopreservation; Holotheria parva; ROS; Spermatozoa; MALE-INFERTILITY; OXYGEN; SEMEN; ANTIOXIDANTS; GENERATION; TREHALOSE; APOPTOSIS; QUALITY; DAMAGE;

D O I：

10.1111/and.13604

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Cryopreservation is the most effective method for preserving semen for a long period of time. However, during the freeze-thaw process, production of reactive oxygen species (ROS) leads to a steep reduction in sperm fertility indices. In this study, we tested the effects of the extract of the coelomic cavity of five Holotheria parva, a marine organism rich in antioxidants, for its ROS-scavenging activity and cryoprotective effects on oxidative stress. Using a total of 50 semen samples, our results demonstrated that doses of 250 and 500 mu g/ml of H. parva coelomic cavity extract significantly increased sperm vitality as compared to the control (p < .05). The addition of 250 mu g/ml of the extract exerted a significant positive effect on sperm motility. Moreover, sperm DNA damage and ROS production were significantly reduced at extract concentrations of 250 and 500 mu g/ml (p < .05). To the best of our knowledge, the results of this study represent the first demonstration of the possibility of improving sperm parameters and reducing ROS production and DNA damage by supplementing sperm freezing media with H. parva coelomic extract. Our results suggested that H. parva coelomic extract could be useful for improving the fertilising ability of frozen-thawed human semen.

引用

页数：8