Structural and optical properties of green spinach extract leaf (Spincia Olercea) prepared with silver nanoparticles as antibacterial by effect of pulsed laser

In this study, silver nanoparticles (AgNPs) were synthesized concurrently by use of a green method and pulsed liquid laser fragmentation (PLFL). The AgNP's, which were generated in an extract solution of Spincia Olercea in order to regulate their size, were focused on the laser wavelength of 532 nm. The influence on the characteristics of manufactured NPs of liquid medium concentration and irradiation time were investigated. While the pattern of X-ray diffraction (XRD) verified AgNPs, the UV-VIS spectrophotometry indicated a large absorption peak at about 406 &458 nm attributable to the typical plasm surface resonance (SPR) peak of the AgNPs acquired. The SPR peak changed to a shorter wavelength by increasing the irradiation period and the concentration of spincia olercea extract. This movement shows the size of the NPs being reduced. The influence from transmission electron microscopy images of PLFL was clearly demonstrated on size reduction. The PLFL method decreased the size of the AgNPs produced to less than 10 nm, depending on the experimental conditions. Bacterial growth was also efficiently inhibited by AgNPs. When combined with a chemical reduction procedure, the PLFL approach has shown to be an effective tool for checking the volumes of NPs.