Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy

被引：19
|
作者：
Fan XiaoJun [1 ]
Zheng Bo [1 ]
Fu YueJun [1 ]
Sun Yi [1 ]
Liang AiHua [1 ]
机构：
[1] Shanxi Univ, Key Lab Chem Biol Mol Engn, Minist Educ, Inst Biotechnol, Taiyuan 030006, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2008年 / 53卷 / 12期
基金：
中国国家自然科学基金;
关键词：
scorpion neurotoxin; BmK IT; recombinant baculovirus; expression; bioassay;
D O I：
10.1007/s11434-008-0242-4
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
An Buthus martensii Karsch Insect Toxin (BmK IT) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1 x 10(7) PIBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6 h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8 h) during a 192 h infection.
引用
收藏
页码：1855 / 1860
页数：6
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