Spectroscopy studies of sandwich-type complex of silver(I) co-ordinated to nuclear fast red and adenine and its analytical applications

被引：2

作者：

Cui, Yi ^{[1
]}

Gong, Yi-Jun ^{[1
]}

Su, Li ^{[1
]}

Wu, Cheng-Ke ^{[1
]}

Pang, Sheng-Bin ^{[1
]}

Feng, Su-Ling ^{[1
]}

机构：

[1] Henan Normal Univ, Minist Educ, Key Lab Green Chem Media & React, Sch Chem & Chem Engn,Collaborat Innovat Ctr Henan, Xinxiang 453007, Peoples R China

来源：

CHEMICAL PAPERS | 2015年 / 69卷 / 04期

关键词：

sandwich-type complex; adenine; nuclear fast red; Ag+; fluorescence quenching; GLASSY-CARBON ELECTRODE; URIC-ACID; GUANINE; SCATTERING; FLUORESCENCE; COMPOSITE; OXIDATION; CYTOSINE; SENSOR; PROBE;

D O I：

10.1515/chempap-2015-0056

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel fluorometric method for the determination of adenine was proposed, based on the fluorescence quenching of a nuclear fast red-silver complex as a small molecule optical probe in the presence of adenine. The characteristic spectra of absorption, resonance Rayleigh scattering (RRS) and the fluorescence of the system were analysed. Adenine, Ag+ and nuclear fast red formed a sandwichtype complex. The composition of the compound and the mechanisms of fluorescence quenching were discussed. The optimal conditions for the reaction and the effects of co-existing substances were also investigated. The quenched fluorescence intensity was proportional to the concentration of adenine in the range of 0.03-0.90 mu g mL(-1), with the limit of detection of 0.023 mu g mL(-1). The method was applied satisfactorily to the determination of adenine in synthetic samples, herring sperm DNA, human sera and vitamin B-4 tablets with recoveries from 95.3 % to 105 %. (c) 2014 Institute of Chemistry, Slovak Academy of Sciences

引用

页码：510 / 517

页数：8