Lipid and aroma composition of black cumin (Nigella sativa L.) seeds from Tunisia

The chemical composition of Tunisian Nigella sativa L. seeds was investigated. The results showed that linoleic acid (C18:2) was the major fatty acid with 65.1% of total fatty acids (TFAs) followed by oleic acid (C18:1) with 12.7% of TFAs. Neutral lipids (NLs) were mainly composed of triacylglycerols with 98.4% of total NLs. Polar lipids were mainly composed of phosphatidylcholine as the major phospholipid subclass, whereas digalactosyldiacylglycerol was the major galactolipid. Total sterols (TSs) represented 2.2% of the fixed oil and were composed of (-sitosterol as the major sterol representing 60.2% of the TSs. The results indicated that the sterols were mainly present in the esterified and in the free forms with 51.2 and 36.1%, respectively, of TS content. Finally, the aroma composition of the volatile oil from oleoresin exhibited the presence of many bioactive compounds such as p-cymene as the major component followed by ocimene, alpha-thujene, octen-3-ol with appreciable rates of 1,8-cineole and thymol.