Phenotypic identification and differentiation of Lactococcus strains isolated from animals

被引:34
|
作者
Pot, B
Devriese, LA
Ursi, D
Vandamme, P
Haesebrouck, F
Kersters, K
机构
[1] STATE UNIV GHENT,FAC VET MED,LAB VET BACTERIOL & MYCOL,B-9000 GHENT,BELGIUM
[2] UNIV ANTWERP,DEPT MED MICROBIOL,B-2020 ANTWERP,BELGIUM
关键词
identification; taxonomy; Lactococcus; domestic animals; subclinical mastitis; SDS-PAGE; antibiotic resistances;
D O I
10.1016/S0723-2020(96)80047-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Lactococcus lactis subsp. lactis, Lactococcus garviene and Lactococcus raffinolactis were identified in samples from different domestic animal species, mainly in tonsils of ruminants, dogs and cats, and milk samples from cases of subclinical mastitis in dairy cows. Species differentiation was mainly based on SDS-PAGE analysis of whole-cell protein patterns. A comparison was made with results of biochemical activity tests and sensitivity patterns to clindamycin and oxacillin. These results were used to construct an improved identification scheme for lactococci from animal origin. By SDS-PAGE analysis of whole-cell proteins, Enterococcus seriolicida was shown to be closely related to Lactococcus garviene.
引用
收藏
页码:213 / 222
页数:10
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