Optical Tweezers calibration: a quantitative tool for local viscosity investigation

Optical tweezers use the gradient force created by tightly focused single laser beam to trap dielectric microparticles. While this technique has been used for over 20 years to manipulate particles without mechanical contact, it is only recently that accurate and quantitative photonic forces measurements have been considered. Moreover of great relevance has resulted the monitoring of Brownian motion of particles confined in optical traps since that provides precious information on local visco-elastic properties of the sorrounding fluid. In this work we consider the still open question concerning the calibration of an optical tweezers which represents the key point for any absolute measurements. In particular, we discuss a novel method to calibrate a quadrant photodiode used as sensor position in the forward scattering scheme. The voltage signals provided by the quadrant photodiode are converted in length units by comparing them with the absolute bead positions measured by means of a calibrated CCD camera. Finally we briefly discuss how calibrated optical tweezers, combined with thermal analysis of the Brownian motion, are potentially of great relevance for microrheological studies of complex fluids.