Detection of Apple Hammerhead Viroid, Apple Luteovirus 1 and Citrus Concave Gum-Associated Virus in Apple Propagation Materials and Orchards in the Czech Republic and Hungary

被引:2
|
作者
Varallyay, Eva [1 ]
Pribylova, Jaroslava [2 ]
Galbacs, Zsuzsanna Nagyne [1 ]
Jahan, Almash [1 ]
Varga, Tunde [1 ]
Spak, Josef [2 ]
Lenz, Ondrej [2 ]
Franova, Jana [2 ]
Sedlak, Jiri [3 ]
Koloniuk, Igor [2 ]
机构
[1] Hungarian Univ Agr & Life Sci, Inst Plant Protect, Dept Plant Pathol, Genom Res Grp, Szent Gyorgyi Albert St 4, H-2100 Godollo, Hungary
[2] Czech Acad Sci, Biol Ctr, Inst Plant Mol Biol, Branisovska 31, Ceske Budejovice 37005, Czech Republic
[3] Res & Breeding Inst Pomol Holovousy Ltd, Holovousy 129, Holovousy 50801, Czech Republic
来源
VIRUSES-BASEL | 2022年 / 14卷 / 11期
关键词
apple; virus; viroid; rootstock; aphid; transmission; HTS; 1ST REPORT; DIAGNOSIS; RNA;
D O I
10.3390/v14112347
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Grafting cultivars onto rootstocks is a widely used practice by the apple industry predominantly aimed at faster fruit bearing. Using high-throughput sequencing, we revealed the presence of recently described viral agents, namely apple hammerhead viroid (AHVd), apple luteovirus 1 (ALV-1), and citrus concave gum-associated virus (CCGaV), in germplasm collections and production orchards in the Czech Republic and Hungary. The HTS results were validated with RT-(q)PCR, and Northern blotting techniques. To obtain further insight about the presence of these agents, RT-PCR based surveys were carried out and showed their widespread presence alone or in mixed infections. The pathogens were present both in production areas and in feral samples. In addition, rootstock-to-scion transmission of ALV-1 and CCGaV was confirmed using commercial rootstock materials. Phylogenetic relationships based on partial sequences of distinct variants were also investigated. Furthermore, the rosy apple aphid was found to be ALV-1-positive, suggesting that it might be a potential vector of the virus.
引用
收藏
页数:20
相关论文
共 3 条
  • [1] Assessing the de novo assemblers: a metaviromic study of apple and first report of citrus concave gum-associated virus, apple rubbery wood virus 1 and 2 infecting apple in India
    Khan, Zainul A.
    Sharma, Susheel Kumar
    Gupta, Nitika
    Diksha, Damini
    Thapa, Pooja
    Shimray, Mailem Yazing
    Prajapati, Malyaj R.
    Nabi, Sajad U.
    Watpade, Santosh
    Verma, Mahendra K.
    Baranwal, Virendra K.
    BMC GENOMICS, 2024, 25 (01):
  • [2] First report of Citrus concave gum-associated virus (CCGaV) on apple (Malus spp.) in South Africa
    Pietersen, Gerhard
    Morgan, Seamus
    Read, David
    JOURNAL OF PLANT PATHOLOGY, 2024, 106 (03) : 1397 - 1398
  • [3] Identification and Characterization of Citrus Concave Gum-Associated Virus Infecting Citrus and Apple Trees by Serological, Molecular and High-Throughput Sequencing Approaches
    Minutolo, Maria
    Cinque, Maria
    Chiumenti, Michela
    Di Serio, Francesco
    Alioto, Daniela
    Navarro, Beatriz
    PLANTS-BASEL, 2021, 10 (11):