Clematomandshurica saponin E, a new triterpenoid saponin from Clematis mandshurica

A new triterpenoid saponin, clematomandshurica saponin E, together with four known saponins were isolated and characterized from the roots and rhizomes of Clematis mandshurica (Ranunculaceae), a commonly used traditional Chinese medicine with anti-inflammatory and antirheumatoid activities. On the basis of spectroscopic analysis, including HR-ESI-MS, IR, 1D, and 2D NMR spectral data and hydrolysis followed by chromatographic analysis, the structure of the new triterpenoid saponin was elucidated as 3-O--l-rhamnopyranosyl-(16)--d-glucopyranosyl-(14)--d-glucopyranosyl-(14)--d-ribopyranosyl-(13)--l-rhamnopyranosyl-(12)--l-arabinopyranosyl oleanolic acid 28-O--d-glucopyranosyl-(16)--d-glucopyranoside.