Optical clearing in transcutaneous Raman spectroscopy of murine cortical bone tissue

被引：31
|
作者：
Schulmerich, Matthew V. [1 ]
Cole, Jacqueline H. [1 ]
Dooley, Kathryn A. [1 ]
Morris, Michael D. [1 ]
Kreider, Jaclynn M. [2 ]
Goldstein, Steven A. [2 ]
机构：
[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Sch Med, Dept Orthopaed Surg, Orthopaed ResLab, Ann Arbor, MI 48109 USA
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2008年 / 13卷 / 02期
关键词：
transcutaneous; optical clearing; Raman spectroscopy; bone; fiber optic probe;
D O I：
10.1117/1.2892687
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of optical clearing with glycerol on the Raman spectra of bone tissue acquired transcutaneously on right and left tibiae from four mice is studied. Multiple transcutaneous measurements are obtained from each limb; glycerol is then applied as an optical clearing agent, and additional transcutaneous measurements are taken. Glycerol reduces the noise in the raw spectra (p = 0.0037) and significantly improves the cross-correlation between the recovered bone factor and the exposed bone measurement in a low signal-to-noise region of the bone spectra (p=0.0245). (C) 2008 Society of Photo-Optical Instrumentation Engineers.
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页数：7
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