Isolation and characterization of rabbit caecal pectinolytic bacteria

被引:18
|
作者
Sirotek, K [1 ]
Marounek, M [1 ]
Rada, V [1 ]
Benda, V [1 ]
机构
[1] Acad Sci Czech Republ, Inst Anim Physiol & Genet, CR-10400 Prague 10, Czech Republic
关键词
D O I
10.1007/BF02825893
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Two hundred and thirty colonies from the caecal contents of six rabbits were picked up and, after a 2-d incubation, were microscopically characterized using Gram staining. Large Gram-negative (34 %) and small Gram-negative (30 %) irregular rods. Gram-negative (27 %) and Gram-positive (8 %) cocci were found. Eleven isolates (Bacteroides ovatus (6 strains), B. thetaiotamicron, B. caccae, B. stercoris, B. capillosus and Capnocytophaga ochracea) were identified using commercial tests for measuring their catalase activity, metabolite production, etc., and testing their growth in 20 % bile. Bacteria belonging to the genus Bacteroides were demonstrated to be the principal pectinolytic organisms in the rabbit caecum.
引用
收藏
页码:79 / 82
页数:4
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