Characterization of a semidominant dwarfing PROCERA allele identified in a screen for CRISPR/Cas9-induced suppressors of loss-of-function alleles

被引：5
|
作者：
Zhu, Zhiguo [1 ,2 ,4 ]
Kang, Xiaojun [1 ,2 ]
Lor, Vai S. [1 ,2 ,5 ]
Weiss, David [3 ]
Olszewski, Neil [1 ,2 ]
机构：
[1] Univ Minnesota, Dept Plant & Microbial Biol, St Paul, MN 55108 USA
[2] Univ Minnesota, Microbial & Plant Genom Inst, St Paul, MN 55108 USA
[3] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Inst Plant Sci & Genet Agr, Rehovot, Israel
[4] Chongqing Univ, Bioengn Coll, Lab Mol Biol Tomato, Chongqing, Peoples R China
[5] Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA
来源：
PLANT BIOTECHNOLOGY JOURNAL | 2019年 / 17卷 / 02期
关键词：
mutagenesis; CRISPR/cas9; gain-of-function mutant; PROCERA; dwarf; reduced GA sensitivity; semidominant; seed germination; GIBBERELLIN; RECEPTOR; MUTANT; GENE;
D O I：
10.1111/pbi.13027
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
引用
收藏
页码：319 / 321
页数：3
相关论文
共 39 条
  • [1] CRISPR-Cas9-mediated loss-of-function screens
    Liu, Jia-qing
    Li, Tao
    FRONTIERS IN LIFE SCIENCE, 2019, 12 (01): : 1 - 13
  • [2] GENOME-WIDE CRISPR/Cas9 LOSS-OF-FUNCTION SCREEN IDENTIFIED THE VULNERABILITY OF HBV-RELATED LIVER CANCER
    Fukuoka, Makoto
    Kodama, Takahiro
    Hikita, Hayato
    Sung, Jihyun
    Shimoda, Akiyoshi
    Shigeno, Satoshi
    Fukutomi, Keisuke
    Murai, Kazuhiro
    Tahata, Yuki
    Yamada, Ryoko
    Sakamori, Ryotaro
    Tatsumi, Tomohide
    Takehara, Tetsuo
    HEPATOLOGY, 2021, 74 : 507A - 507A
  • [3] Protocol for performing pooled CRISPR-Cas9 loss-of-function screens
    Mathiowetz, Alyssa J.
    Roberts, Melissa A.
    Morgens, David W.
    Olzmann, James A.
    Li, Zhipeng
    STAR PROTOCOLS, 2023, 4 (02):
  • [4] Generation and Characterization of a CRISPR/Cas9-Induced 3-mst Deficient Zebrafish
    Katsouda, Antonia
    Peleli, Maria
    Asimakopoulou, Antonia
    Papapetropoulos, Andreas
    Beis, Dimitris
    BIOMOLECULES, 2020, 10 (02)
  • [5] Effective screen of CRISPR/Cas9-induced mutants in rice by single-strand conformation polymorphism
    Zheng, Xuelian
    Yang, Shixin
    Zhang, Dengwei
    Zhong, Zhaohui
    Tang, Xu
    Deng, Kejun
    Zhou, Jianping
    Qi, Yiping
    Zhang, Yong
    PLANT CELL REPORTS, 2016, 35 (07) : 1545 - 1554
  • [6] Effective screen of CRISPR/Cas9-induced mutants in rice by single-strand conformation polymorphism
    Xuelian Zheng
    Shixin Yang
    Dengwei Zhang
    Zhaohui Zhong
    Xu Tang
    Kejun Deng
    Jianping Zhou
    Yiping Qi
    Yong Zhang
    Plant Cell Reports, 2016, 35 : 1545 - 1554
  • [7] Characterization of CRISPR/Cas9-induced genomic alterations and AAV targeted integration in human HSPC
    Tachtsidi, A.
    Corre, G.
    Amendola, M.
    HUMAN GENE THERAPY, 2022, 33 (23-24) : A143 - A144
  • [8] Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo
    Gandhi, Shashank
    Piacentino, Michael L.
    Vieceli, Felipe M.
    Bronner, Marianne E.
    DEVELOPMENTAL BIOLOGY, 2017, 432 (01) : 86 - 97
  • [9] Characterization and Quantification of CRISPR/Cas9-Induced Genomic Alterations and AAV Targeted Integration in Human HSPC
    Tachtsidi, Alexandra
    Corre, Guillaume
    Lacombe, Laurie
    Amendola, Mario
    MOLECULAR THERAPY, 2023, 31 (04) : 267 - 268
  • [10] CRISPR/Cas9-induced DNA breaks trigger crossover, chromosomal loss, and chromothripsis-like rearrangements
    Samach, Aviva
    Mafessoni, Fabrizio
    Gross, Or
    Melamed-Bessudo, Cathy
    Filler-Hayut, Shdema
    Dahan-Meir, Tal
    Amsellem, Ziva
    Pawlowski, Wojciech P.
    Levy, Avraham A.
    PLANT CELL, 2023, 35 (11): : 1957 - 1972
1234