Determination of Fumonisin B1 and Fumonisin B2 in Formula Feeds for Livestock and Poultry by High Performance Liquid Chromatography-Tandem Mass Spectrometry

A method was developed for the determination of fumonisin B-1(FB1) and fumonisin B-2(FB2) in livestock and poultry formula feeds by high performance liquid chromatography. tandem mass spectrometry (LC-MS/MS. After extracted by acetonitrile. water (50: 50, V /V) and purified with MAX solid phase extraction column, the fumonisins were separated by Thermo C-18 (100 mmx2. 1 mm, 5 mu m) column with 0. 1% formic acid in water and methanol as the mobile phase. Multiple reaction monitoring (MRM) was used to acquire mass spectrometric data under electrospray positive ionization mode (ESI+). The results showed that the linear correlation coefficients (R-2) of fumonisin FB1 and FB2 were all greater than 0. 999 in the range of 1-500 mu g/L. The limits of quantitation (LOQ) were 0. 098 and 0. 197 mu g/L, and the limits of detection (LOD) were 0. 328 and 0. 656 mu g/L, respectively. At different spiked levels, the recoveries of FB1 and FB2 were ranged from 89. 7% to 95. 1%, and the relative standard deviation (RSD) was ranged from 3. 2% to 8. 6%. Additionally, the detection rate reached 98. 11% screening through the established method in the 106 livestock and poultry formula feeds collected from markets. This result indicates that the method is suitable for accurate quantitative analysis of FB1 and FB2 in different complicated livestock and poultry formula feeds.